Increased Nitrosative Stress in Eosinophilic Pneumonia
Author Information
Author(s): Furukawa Kanako, Sugiura Hisatoshi, Matsunaga Kazuto, Ichikawa Tomohiro, Koarai Akira, Hirano Tsunahiko, Yanagisawa Satoru, Minakata Yoshiaki, Akamatsu Keiichiro, Kanda Masae, Nishigai Manabu, Ichinose Masakazu
Primary Institution: Wakayama Medical University School of Medicine
Hypothesis
Nitrosative stress markers including Calv, inducible type of NO synthase (iNOS), and 3-nitrotyrosine (3-NT) are upregulated in eosinophilic pneumonia (EP).
Conclusion
Excessive nitrosative stress occurs in eosinophilic pneumonia, and Calv could be a marker of disease activity.
Supporting Evidence
- The Calv levels in the EP group were significantly higher than those in healthy subjects and IPF patients.
- More iNOS and 3-NT positive cells were observed in the EP group compared to healthy subjects and IPF patients.
- Corticosteroid treatment significantly reduced both the FENO and Calv levels.
- The magnitude of reduction in the Calv levels had a significant positive correlation with peripheral blood eosinophil counts.
Takeaway
People with eosinophilic pneumonia have too much of a harmful substance called nitric oxide in their lungs, which can make them sick. A test that measures this can help doctors see how bad the disease is.
Methodology
Exhaled nitric oxide levels were measured in healthy subjects and patients with idiopathic pulmonary fibrosis and eosinophilic pneumonia, along with iNOS expression and 3-NT formation assessed in BALf cells.
Limitations
The study could not collect BALf samples from all groups, and the sample size was limited.
Participant Demographics
10 healthy subjects, 13 patients with idiopathic pulmonary fibrosis, and 13 patients with eosinophilic pneumonia; all subjects were never- or ex-smokers.
Statistical Information
P-Value
p<0.01
Statistical Significance
p<0.01
Digital Object Identifier (DOI)
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