Study of Fungal Pectinase from Different Plants
Author Information
Author(s): Mwaheb Mai Ali, El-Aziz Basant Mohamed Abd, Abd-Elhalim Basma T., El-Kassim Nabil Abo, Radwan Tharwat E. E.
Primary Institution: Fayoum University, Ain Shams University
Hypothesis
Can fungi from various plant rhizospheres produce effective pectinase enzymes?
Conclusion
The study identified several fungal isolates that produce high levels of pectinase, which can be used in various industrial applications.
Supporting Evidence
- The highest pectinase degrading index was observed for FB5, FJ2, and FW1 isolates.
- Pectinase production was maximized at specific pectin concentrations and temperatures.
- The enzyme's molecular weight was approximately 35 kDa.
- Biocompatibility was assessed using a human skin cell line with an IC50 of 151.86 U/ml.
- The enzyme was effectively used as a bioscouring agent in textiles.
Takeaway
Scientists found that certain fungi from plants can make a special enzyme called pectinase, which helps break down pectin, a substance in fruits and plants.
Methodology
The study involved isolating fungi from soil samples, screening for pectinase production, and optimizing conditions for enzyme production.
Limitations
The study was limited to specific plant rhizospheres in Fayoum Governorate, Egypt.
Statistical Information
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
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