Nedocromil Sodium Reduces HLA-DR and ICAM-1 Expression in Bronchial Epithelial Cells
Author Information
Author(s): O. Sacco, S. Lantero, L. Scarso, V. Frangova, V. Ottolini, G. A. Rossi
Primary Institution: Istituto G. Gaslini, Genova, Italy
Hypothesis
Mononuclear cell products could increase the expression of HLA-DR and ICAM-1 molecules in bronchial epithelial cells.
Conclusion
Nedocromil sodium effectively downregulates the expression of HLA-DR and ICAM-1 on bronchial epithelial cells.
Supporting Evidence
- Nedocromil sodium inhibited HLA-DR and ICAM-1 expression in a dose-dependent manner.
- Dexamethasone also inhibited HLA-DR and ICAM-1 expression but was less effective than nedocromil.
- Both drugs showed a significant synergistic effect at lower concentrations.
Takeaway
This study shows that certain cells can make lung cells express more markers that help in inflammation, but a medicine called nedocromil can help reduce that expression.
Methodology
Human bronchial epithelial cells were cultured and treated with PHA-activated blood mononuclear cell conditioned media and nedocromil sodium to evaluate the expression of HLA-DR and ICAM-1.
Participant Demographics
Healthy donors provided blood samples for mononuclear cell isolation.
Statistical Information
P-Value
p<0.01 for HLA-DR and p<0.05 for ICAM-1
Statistical Significance
p<0.05
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