c-Myc Regulates Self-Renewal in Bronchoalveolar Stem Cells
Author Information
Author(s): Dong Jie, Sutor Shari, Jiang Guoqian, Cao Yajun, Asmann Yan W., Wigle Dennis A.
Primary Institution: Mayo Clinic, Rochester, Minnesota, United States of America
Hypothesis
c-Myc plays a critical role in maintaining the self-renewal capacity of lung bronchoalveolar stem cells.
Conclusion
c-Myc is essential for the self-renewal of bronchoalveolar stem cells, as its depletion leads to decreased proliferation and increased cell death.
Supporting Evidence
- c-Myc expression was upregulated in BASCs and downregulated during lung organogenesis.
- Depletion of c-Myc in BASCs resulted in decreased proliferation and increased cell death.
- 250 mRNAs and 17 miRNAs were dynamically regulated in c-Myc depleted BASCs.
Takeaway
c-Myc is like a superhero for certain lung cells, helping them grow and stay alive. If we take away c-Myc, these cells struggle to survive.
Methodology
BASCs were isolated from adult mouse lung using FACS, and their self-renewal and differentiation were assessed through immunostaining and gene expression profiling.
Participant Demographics
Adult mice were used for the study.
Statistical Information
P-Value
p<0.05
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
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