Gene Expression Changes in Buffalo Cow Follicles During Ovulation
Author Information
Author(s): Rao Jyotsna U., Shah Kunal B., Puttaiah Jayaram, Rudraiah Medhamurthy
Primary Institution: Indian Institute of Science, Bangalore, India
Hypothesis
Increased IGF-I concentration affects gene expression in preovulatory follicles of buffalo cows.
Conclusion
Increased intrafollicular IGF-I levels significantly alter gene expression associated with steroidogenesis and apoptosis during the periovulatory period.
Supporting Evidence
- Increased IGF-I levels caused changes in expression of genes associated with steroidogenesis.
- 450 genes were differentially expressed at 1 hour post LH surge.
- 111 genes were differentially expressed at 22 hours post LH surge.
- Expression of BCL-2 was upregulated post peak LH surge.
- FKHR expression was downregulated after the onset of peak LH surge.
Takeaway
This study shows that when buffalo cows have more IGF-I, it changes how their eggs develop and can help them get pregnant better.
Methodology
Microarray analysis was performed on granulosa cells collected from preovulatory follicles at various time points post LH surge.
Limitations
The study did not analyze protein expression for many differentially expressed genes due to limited cell availability.
Participant Demographics
Non-lactating buffalo cows
Statistical Information
P-Value
p<0.05
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
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