Focal distortion of the nuclear envelope by huntingtin aggregates revealed by lamin immunostaining
2008
Huntingtin Aggregates Distort the Nuclear Envelope
publication
Evidence: moderate
Author Information
Author(s): Chapple J. Paul, Bros-Facer Virginie, Butler Rachel, Gallo Jean-Marc
Primary Institution: MRC Centre for Neurodegeneration Research, King’s College London
Hypothesis
The study investigates how huntingtin aggregates affect the nuclear envelope in cells.
Conclusion
Huntingtin aggregates cause a focal distortion of the nuclear envelope in transfected cells.
Supporting Evidence
- Huntingtin aggregates in a juxtanuclear position were associated with a clear focal distortion in the nuclear envelope.
- Lamin B1 distribution was not altered by aggregates of polyglutamine-expanded ataxin-1.
- Immunocytochemistry for lamin B1 can be used to monitor the state of the nuclear envelope in experimental studies.
Takeaway
When certain proteins related to Huntington's disease clump together, they can push against the nuclear envelope, changing its shape.
Methodology
The study used immunostaining for lamin B1 and laser confocal microscopy to analyze the nuclear envelope in transfected CHO cells.
Digital Object Identifier (DOI)
Want to read the original?
Access the complete publication on the publisher's website