Contribution of the Caspase Gene Sequence Diversification to the Specifically Antiviral Defense in Invertebrate
2011

How Shrimp Use a Special Gene to Fight Viruses

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Author Information

Author(s): Zhi Bin Wang, Wang Lei, Wang Guangyi, Zhang Xiaobo

Primary Institution: Key Laboratory of Conservation Biology for Endangered Wildlife of the Ministry of Education, Zhejiang University

Hypothesis

The study investigates the role of the PjCaspase gene sequence diversity in the antiviral defense of shrimp against the white spot syndrome virus (WSSV).

Conclusion

The PjCaspase gene containing a specific fragment is crucial for the antiviral defense of shrimp against WSSV, as it modulates apoptosis in response to the virus.

Supporting Evidence

  • The PjCaspase gene from WSSV-resistant shrimp contained a unique fragment that enhanced antiviral responses.
  • Silencing the PjCaspase gene led to a significant increase in WSSV copies in shrimp.
  • Overexpression of the PjCaspase gene resulted in a significant decrease in WSSV replication.

Takeaway

Shrimp have a special gene that helps them fight off viruses by making their cells die when infected, which stops the virus from spreading.

Methodology

The study involved comparing the PjCaspase gene sequences from WSSV-free and WSSV-resistant shrimp and conducting RNA interference assays to assess the gene's role in apoptosis.

Limitations

The study primarily focuses on one gene in shrimp and may not represent the entire immune response of invertebrates.

Participant Demographics

The study involved shrimp from various locations in China.

Statistical Information

P-Value

p<0.01

Statistical Significance

p<0.01

Digital Object Identifier (DOI)

10.1371/journal.pone.0024955

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