Regulation of the CRELD2-ALG12 Gene Pair Under ER Stress
Author Information
Author(s): Oh-hashi Kentaro, Koga Hisashi, Ikeda Shun, Shimada Kiyo, Hirata Yoko, Kiuchi Kazutoshi
Primary Institution: Gifu University
Hypothesis
The CRELD2 and ALG12 genes are regulated by a common bidirectional promoter influenced by ER stress.
Conclusion
The study reveals that the CRELD2 and ALG12 genes are regulated by multiple transcription factors under ER stress conditions.
Supporting Evidence
- CRELD2 and ALG12 mRNAs were induced in Neuro2a cells by ER stress inducers.
- The ERSE motif in the promoter region is crucial for the transcriptional regulation of CRELD2.
- Three suppressive sites in the intergenic region were identified that affect promoter activity.
Takeaway
This study shows that two genes, CRELD2 and ALG12, work together and can be turned on by stress in cells, helping them deal with problems.
Methodology
Microarray analysis and luciferase reporter assays were used to evaluate gene expression and promoter activity.
Limitations
The study does not explore the full range of potential regulatory mechanisms affecting the gene pair.
Digital Object Identifier (DOI)
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