Timing Is Critical for Effective Glucocorticoid Receptor Mediated Repression of the cAMP-Induced CRH Gene
2009
Timing of CRH Gene Repression by Glucocorticoids
publication
Evidence: moderate
Author Information
Author(s): Siem van der Laan, E. Ronald Meijer, Onno C. de Kloet
Primary Institution: Leiden University Medical Center
Hypothesis
Effective repression of the cAMP-induced hCRH proximal promoter depends on the relative timing of GR activation.
Conclusion
A critical time-window exists for effective repression of the CRH gene by glucocorticoids.
Supporting Evidence
- Glucocorticoid treatment can significantly repress CRH-promoter activity.
- Timing of GR activation affects the level of repression of the CRH gene.
- A 10-minute delay in GR activation leads to a significant loss of repression.
Takeaway
If you wait too long to give a certain medicine after a signal, it won't work as well. Timing is really important!
Methodology
The study used the At-T20 cell-line to investigate the timing of glucocorticoid receptor activation and its effect on CRH gene transcription.
Limitations
The study is based on a cell-line model, which may not fully replicate in vivo conditions.
Statistical Information
P-Value
p<0.05
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
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