Evaluating the Toxicity of Citrinin Using Yeast Microarrays
Author Information
Author(s): Iwahashi Hitoshi, Kitagawa Emiko, Suzuki Yoshiteru, Ueda Youji, Ishizawa Yo-hei, Nobumasa Hitoshi, Kuboki Yoshihide, Hosoda Hiroshi, Iwahashi Yumiko
Primary Institution: National Institute of Industrial Science and Technology, AIST
Hypothesis
What is the genomic response of yeast cells to citrinin exposure?
Conclusion
Citrinin treatment induces oxidative stress in yeast cells, but is less toxic than patulin.
Supporting Evidence
- Citrinin inhibited growth of yeast cells at concentrations higher than 100 ppm.
- Oxidative stress response genes were significantly induced by citrinin treatment.
- The expression profiles from both microarray types were similar, but the Oligo DNA microarray showed better reproducibility.
Takeaway
Citrinin is a harmful substance made by fungi that can hurt yeast cells, but it's not as bad as another toxin called patulin.
Methodology
The study used ORF and Oligo DNA microarrays to monitor mRNA expression in yeast cells exposed to citrinin.
Limitations
The Oligo DNA microarray did not completely overcome cross hybridization.
Statistical Information
P-Value
p<0.01
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
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