Functional Tat Translocases from Different Bacterial Species
Author Information
Author(s): Matthew G. Hicks, David Guymer, Grant Buchanan, David A. Widdick, Isabelle Caldelari, Ben C. Berks, Tracy Palmer
Primary Institution: John Innes Centre
Hypothesis
Can tat genes from different eubacterial species compensate for the absence of the cognate E. coli tat gene?
Conclusion
The study found that TatA proteins from Streptomyces coelicolor showed the highest level of cross-species complementation in E. coli.
Supporting Evidence
- TatA proteins from Streptomyces coelicolor were able to form functional translocases with E. coli components.
- Pseudomonas syringae TatA and TatB proteins showed limited functionality.
- Aquifex aeolicus TatA proteins had detectable but low functionality.
Takeaway
Scientists tested proteins from different bacteria to see if they could help E. coli transport other proteins. They found that one type of protein worked best.
Methodology
The study involved expressing tat genes from three bacterial species in E. coli strains lacking tat components and assessing their functionality through growth and enzyme activity tests.
Limitations
The study did not explore all possible bacterial species or conditions that might affect Tat functionality.
Digital Object Identifier (DOI)
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