Synthesis of Three Novel Oligosaccharides Using Kojibiose Phosphorylase
Author Information
Author(s): Takahashi Natsuko, Fukushi Eri, Onodera Shuichi, Benkeblia Noureddine, Nishimoto Tomoyuki, Kawabata Jun, Shiomi Norio
Primary Institution: Rakuno Gakuen University
Hypothesis
The purpose of this study was to synthesize novel oligosaccharides using kojibiose phosphorylase.
Conclusion
Three novel oligosaccharides were successfully synthesized and their structures were fully determined.
Supporting Evidence
- Three novel oligosaccharides were synthesized from xylosylfructoside and β-D-G1P using kojibiose phosphorylase.
- The structures of the synthesized oligosaccharides were confirmed using NMR and mass spectrometry.
- The synthesis process involved glucosyltransfer reactions catalyzed by the enzyme.
Takeaway
Scientists made three new types of sugar by mixing two ingredients with a special enzyme, and they figured out what these new sugars looked like.
Methodology
The oligosaccharides were synthesized by glucosyltransfer from β-D-glucose 1-phosphate to xylosylfructoside using kojibiose phosphorylase, followed by structural characterization using NMR and mass spectrometry.
Digital Object Identifier (DOI)
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