Impact of Protein Properties on Detecting Tumor-Derived Proteins in Blood
Author Information
Author(s): Fang Qiaojun, Kani Kian, Faca Vitor M., Zhang Wenxuan, Zhang Qing, Jain Anjali, Hanash Sam, Agus David B., McIntosh Martin W., Mallick Parag
Primary Institution: Fred Hutchinson Cancer Research Center
Hypothesis
How do protein stability, cellular localization, and abundance affect the detection of tumor-derived proteins in plasma?
Conclusion
The study found that extracellular and stable proteins are more likely to be detected in plasma than intracellular proteins.
Supporting Evidence
- 20% of proteins identified in plasma were tumor-derived.
- Extracellular proteins were seven times more likely to be detected in plasma than intracellular proteins.
- Doubling the spectral count increased detection rate by only 50%.
- Stable proteins were significantly more likely to be identified in plasma than less stable proteins.
Takeaway
This study shows that proteins from tumors can be found in blood, and those that are secreted and stable are easier to detect.
Methodology
Proteomic profiling was performed on tumors and plasma from xenograft mouse models, followed by statistical analysis of protein properties.
Limitations
The model may not fully reflect human disease due to differences in tumor burden and localization.
Participant Demographics
Mice with human tumor xenografts were used in the study.
Statistical Information
P-Value
p<0.001
Statistical Significance
p<0.001
Digital Object Identifier (DOI)
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