Glutathione S-transferase in Ovarian Tumours
Author Information
Author(s): A.G.J. van der Zee, B. van Ommen, C. Meijer, H. Hollema, P.J. van Bladeren, E.G.E de Vries
Primary Institution: University Hospital, Groningen; TNO Toxicology and Nutrition Institute, Netherlands
Hypothesis
The study aims to identify and quantify GST isoenzymes in benign and malignant ovarian tumours before and after chemotherapy.
Conclusion
The study found that GST activity and GST pi levels were lower in malignant ovarian tumours after chemotherapy compared to untreated tumours.
Supporting Evidence
- Mean GST activity was decreased in malignant ovarian tumours after chemotherapy compared to untreated tumours.
- GST isoenzyme patterns were identical in benign and malignant tumours before and after chemotherapy.
- No relation was found between GST pi amount and response to chemotherapy in untreated ovarian tumours.
Takeaway
This study looked at how certain enzymes in ovarian tumours change before and after chemotherapy, finding that these enzymes were lower after treatment.
Methodology
The study measured GST activity and isoenzyme composition in tumour samples using HPLC and other biochemical techniques.
Potential Biases
The study may have biases due to the small number of paired samples and the timing of sample collection after chemotherapy.
Limitations
The study had a small sample size and varied histological types of tumours, which may affect the generalizability of the results.
Participant Demographics
Patients included 4 with benign cystadenomas and 20 with untreated ovarian adenocarcinoma, with 16 patients having specimens obtained after chemotherapy.
Statistical Information
P-Value
p<0.05
Statistical Significance
p<0.05
Want to read the original?
Access the complete publication on the publisher's website