Fluid Shear Stress Affects Glioma Cell Migration and Invasion
Author Information
Author(s): Qazi, Henry, Shi, Zhong-Dong, Tarbell, John M.
Primary Institution: Department of Biomedical Engineering, City College of New York, City University of New York
Hypothesis
How does fluid shear stress influence the migratory activity and invasive potential of glioma cells?
Conclusion
Fluid shear stress in the tumor microenvironment reduces glioma invasion by modulating cell motility and matrix metalloproteinase levels.
Supporting Evidence
- U87 and CNS-1 cell lines showed up to 92% and 58% suppression in migratory activity due to shear stress.
- MMP inhibition experiments demonstrated that glioma cells depend on MMP activity for invasion.
- Flow-induced migration trends were consistent with reported invasive potentials of implanted gliomas.
Takeaway
When glioma cells are exposed to fluid flow, they move less and invade less because the flow changes how they behave and affects certain proteins they need to move.
Methodology
A 3D Modified Boyden chamber model was used to study the effects of fluid shear stress on glioma cell migration, utilizing three cell lines and measuring MMP activity.
Limitations
The study does not account for other in vivo factors such as hypoxia and stromal cell interactions that may influence tumor behavior.
Statistical Information
P-Value
p<0.005
Statistical Significance
p<0.005
Digital Object Identifier (DOI)
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