Effects of Ischemia and Blood Flow Recovery in Mouse Models
Author Information
Author(s): Al-Mubarak Husain A, Alamri Talal M, Aljabab Saif A, Atteya Mohammad, Quan Adrian, Teoh Hwee, Shukla Praphulla C, Verma Subodh, Aldahmash Abdullah, Aljabri Badr, Napoli Claudio, Al-Omran Mohammed
Primary Institution: King Saud University
Hypothesis
The study aimed to investigate the duration of ischemia and blood flow recovery in mouse models of partial femoral artery ligation.
Conclusion
The currently described models do not pose major improvements over previously described methods.
Supporting Evidence
- The ligation over needle method showed a mean perfusion decrease of -71.87% immediately post procedure.
- The transfixation method showed a mean perfusion decrease of -70.82% immediately post procedure.
- Both methods resulted in a recovery pattern of ischemia that was independent of the initial postoperative decrease in perfusion.
- The calf muscle was identified as the optimal sample for obtaining representative results.
Takeaway
The researchers looked at how blood flow returns after blocking blood supply in mice, and found that their methods didn't work any better than older ones.
Methodology
Male BALB/c mice were used, and two surgical methods were employed to induce ischemia, followed by Laser Doppler Perfusion Imaging to assess blood flow recovery.
Limitations
The study had a relatively low success rate of 50% for both methods and a limited number of mice that completed the study duration.
Participant Demographics
Male BALB/c mice aged between 11-12 weeks and weighing 23-34 g.
Digital Object Identifier (DOI)
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