Using Quantum Dots in Flow Cytometry
Author Information
Author(s): Ibáñez-Peral Raquel, Bergquist Peter L., Walter Malcolm R., Gibbs Moreland, Goldys Ewa M., Ferrari Belinda
Primary Institution: Macquarie University
Hypothesis
Can quantum dots (QDs) improve detection limits in flow cytometry compared to traditional dyes?
Conclusion
Quantum dots provide a significantly lower detection limit in flow cytometry compared to fluorescein isothiocyanate (FITC).
Supporting Evidence
- Quantum dots can be excited by a wide range of wavelengths, making them versatile for various applications.
- The minimum concentration of quantum dots needed for detection was found to be 100 times less than that of FITC.
- Quantum dots showed lower detection limits even under non-optimal excitation conditions.
Takeaway
Quantum dots are tiny particles that can help scientists see things better in tests, like finding germs in water, because they shine brighter than regular dyes.
Methodology
The study compared the fluorescence intensity of quantum dots and FITC labeled beads using flow cytometry.
Limitations
The optical properties of quantum dots can be affected by their surface chemistry and the conditions under which they are used.
Digital Object Identifier (DOI)
Want to read the original?
Access the complete publication on the publisher's website