Isolation and antisense suppression of flavonoid 3', 5'-hydroxylase modifies flower pigments and colour in cyclamen
2010

Changing Flower Colors in Cyclamen Through Genetic Modification

publication Evidence: moderate

Author Information

Author(s): Boase Murray R, Lewis David H, Davies Kevin M, Marshall Gayle B, Patel Deepa, Schwinn Kathy E, Deroles Simon C

Primary Institution: New Zealand Institute for Plant & Food Research Ltd

Hypothesis

Can the suppression of flavonoid 3', 5'-hydroxylase modify flower pigments and color in cyclamen?

Conclusion

This study successfully modified flower colors in cyclamen by altering anthocyanin profiles through genetic modification.

Supporting Evidence

  • Transgenic lines showed modified flower colors that correlated with reduced levels of F3'5'H transcript.
  • Total anthocyanin concentration decreased by up to 80% in some transgenic lines.
  • A shift in anthocyanin profile from delphinidin-derived to cyanidin-derived pigments was observed.

Takeaway

Scientists changed the colors of cyclamen flowers by tweaking a gene that controls flower pigments, making them look different.

Methodology

Transgenic lines of cyclamen were created using Agrobacterium tumefaciens to suppress the F3'5'H gene, followed by analysis of pigment changes.

Limitations

The study did not achieve complete suppression of F3'5'H, which may have limited the production of certain pigments.

Statistical Information

P-Value

p<0.05

Statistical Significance

p<0.05

Digital Object Identifier (DOI)

10.1186/1471-2229-10-107

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