Molecular monitoring of low grade non-Hodgkin's lymphoma by gene amplification
1991

Molecular Monitoring of Low Grade Non-Hodgkin's Lymphoma

Sample size: 30 publication Evidence: moderate

Author Information

Author(s): T.F. Hickish, H. Purvis, J. Mansil, M. Soukop, D. Cunningham

Primary Institution: Royal Marsden Hospital, Sutton, Surrey; Glasgow Royal Infirmary, Glasgow, Scotland, UK

Hypothesis

Can polymerase chain reaction (PCR) effectively monitor minimal disease in low grade non-Hodgkin's lymphoma?

Conclusion

PCR can be used to upstage disease status in low grade lymphoma and monitor treatment response.

Supporting Evidence

  • PCR analysis detected malignant cells in approximately 50% of patients when bone marrow histology was negative.
  • PCR was able to follow a patient's disease into remission and detect subclinical disease.
  • 80% of patients with positive bone marrow histology had positive PCR results.

Takeaway

Doctors can use a special test called PCR to find tiny amounts of cancer in patients with a type of lymphoma, helping them see if the treatment is working.

Methodology

PCR analysis of bone marrow and blood samples was compared to conventional histology in 30 patients.

Limitations

Some patients' primary tumors were unavailable, making it unclear if they had a bcl-2 rearrangement.

Participant Demographics

Patients diagnosed with working formulation category B or C non-Hodgkin's lymphoma.

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