Effect of Ketamine on the Circadian Clock
2011
Ketamine Influences CLOCK:BMAL1 Function Leading to Altered Circadian Gene Expression
publication
Evidence: moderate
Author Information
Author(s): Marina M. Bellet, Marquis P. Vawter, Blynn G. Bunney, William E. Bunney, Paolo Sassone-Corsi
Primary Institution: University of California Irvine
Hypothesis
How does ketamine affect circadian gene expression?
Conclusion
Ketamine alters circadian rhythms by inhibiting CLOCK:BMAL1 mediated transcription.
Supporting Evidence
- Ketamine modulates CLOCK:BMAL1-mediated transcriptional activation in neuronal cells.
- Ketamine treatment leads to a dose-dependent reduction in circadian transcription of key genes.
- Chromatin-immunoprecipitation analyses show altered recruitment of CLOCK:BMAL1 complex on circadian promoters.
Takeaway
Ketamine can change how our body's internal clock works, which might help treat depression quickly.
Methodology
The study involved treating neuronal cells with ketamine and analyzing its effects on circadian gene expression and CLOCK:BMAL1 recruitment using various assays.
Limitations
The study primarily used in vitro models, which may not fully replicate in vivo conditions.
Statistical Information
P-Value
p<0.01
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
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