miR-143 Regulation of Prostaglandin-Endoperoxidase Synthase 2 in the Amnion: Implications for Human Parturition at Term
Author Information
Author(s): Kim Sun Young, Romero Roberto, Tarca Adi L., Bhatti Gaurav, Lee JoonHo, Chaiworapongsa Tinnakorn, Hassan Sonia S., Kim Chong Jai
Primary Institution: Perinatology Research Branch, National Institute of Child Health and Human Development/National Institutes of Health/Department of Health and Human Services
Hypothesis
This study aims to determine the microRNA expression pattern and its significance in the placental amnion and the reflected amnion in association with labor at term.
Conclusion
The study reveals that miRNA-mediated post-transcriptional regulation of gene expression in the amnion plays an important role in parturition.
Supporting Evidence
- MicroRNA microarray analysis demonstrated differential expression of 32 miRNAs between the placental amnion and the reflected amnion after labor.
- Thirty-one (97%) miRNAs, including miR-143 and miR-145, were down-regulated in the reflected amnion.
- Expression of miR-143 and miR-145 was higher in amnion mesenchymal cells than in amnion epithelial cells.
Takeaway
This study found that certain tiny molecules called miRNAs help control important genes in the amnion, which is crucial for childbirth.
Methodology
MicroRNA microarray, real-time quantitative RT-PCR, and miRNA in situ hybridization analyses were conducted on amnion tissues.
Participant Demographics
{"term_in_labor":11,"term_no_labor":10,"maternal_age":{"term_in_labor":"22 (20–39)","term_no_labor":"29 (19–38)"},"race":{"black":{"term_in_labor":"81.8%","term_no_labor":"60.0%"},"white":{"term_in_labor":"0.0%","term_no_labor":"30.0%"},"hispanic":{"term_in_labor":"9.1%","term_no_labor":"0.0%"},"others":{"term_in_labor":"9.1%","term_no_labor":"10.0%"}}}
Statistical Information
P-Value
p<0.05
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
Want to read the original?
Access the complete publication on the publisher's website