Effect of reconstituted basement membrane components on the growth of a panel of human tumour cell lines in nude mice
1993

Effect of Matrigel on Tumor Growth in Mice

Sample size: 20 publication Evidence: moderate

Author Information

Author(s): P. Topley, D.C. Jenkins, E.A. Jessup, J.N. Stables

Primary Institution: Wellcome Research Laboratories

Hypothesis

Does the co-injection of reconstituted basement membrane components (Matrigel) enhance the growth of human tumor cell lines in nude mice?

Conclusion

Matrigel significantly enhances the growth and tumorigenicity of certain human tumor cell lines in nude mice.

Supporting Evidence

  • Matrigel improved tumor growth rates in three out of four tested cell lines.
  • The growth rate of SW480 xenografts was significantly enhanced when co-injected with Matrigel.
  • A549 cells showed increased tumorigenicity when co-injected with Matrigel.

Takeaway

Matrigel helps tumors grow better in mice, making it easier to study cancer.

Methodology

The study involved co-injecting human tumor cell lines with Matrigel into nude mice and measuring tumor growth and characteristics.

Limitations

The study only tested a limited number of cell lines and did not explore all potential effects of Matrigel.

Participant Demographics

Male athymic CD 1 (nu/nu) mice, aged 4 to 8 weeks.

Statistical Information

P-Value

p<0.01

Statistical Significance

p<0.01

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