Regulation of Peptidoglycan Synthesis by Outer Membrane Proteins
Author Information
Author(s): Typas Athanasios, Banzhaf Manuel, van den Berg van Saparoea Bart, Verheul Jolanda, Biboy Jacob, Nichols Robert J., Zietek Matylda, Beilharz Katrin, Kannenberg Kai, von Rechenberg Moritz, Breukink Eefjan, den Blaauwen Tanneke, Gross Carol A., Vollmer Waldemar
Primary Institution: Department of Microbiology & Immunology, University of California at San Francisco
Hypothesis
How do outer membrane lipoproteins LpoA and LpoB regulate the activity of peptidoglycan synthases PBP1A and PBP1B in E. coli?
Conclusion
LpoA and LpoB are essential for the function of their respective peptidoglycan synthases and play a role in outer membrane constriction during cell division.
Supporting Evidence
- LpoA and LpoB are essential for the function of PBP1A and PBP1B.
- Lpo proteins stimulate the transpeptidase activity of their cognate PBPs.
- LpoB localizes at the midcell during cell division.
- Depletion of Lpo proteins leads to cell lysis.
- Lpo proteins interact specifically with their cognate PBPs.
- Mutants lacking Lpo proteins exhibit synthetic lethality.
- Lpo proteins are evolutionarily restricted to γ-proteobacteria.
Takeaway
This study found that two proteins on the outside of bacteria help the bacteria grow and divide by making sure their cell walls are built correctly.
Methodology
The study used genetic interaction screens and proteomic approaches to identify and characterize the roles of LpoA and LpoB in peptidoglycan synthesis.
Statistical Information
P-Value
p<10−116
Statistical Significance
p<10−116
Digital Object Identifier (DOI)
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