Neuroglycan C Expression in Retinal Degeneration
Author Information
Author(s): Escher Pascal, Cottet Sandra, Aono Saichiko, Oohira Atsuhiko, Schorderet Daniel F.
Primary Institution: Institute for Research in Ophthalmology, Sion, Switzerland
Hypothesis
The study aims to validate and characterize the expression of neuroglycan C during retinal degeneration in Rpe65−/− mice.
Conclusion
NGC expression is induced in the neural retina during retinal degeneration in Rpe65−/− mice, but not in the RPE.
Supporting Evidence
- NGC mRNA expression was induced by over 2 fold in Rpe65−/− retinas versus wild-type levels.
- NGC-I was the major isoform detected in the retina and the RPE.
- NGC protein levels were induced in retinas from 2- and 4-month-old Rpe65−/− mice.
Takeaway
This study found that a protein called neuroglycan C increases in the eyes of mice with a specific genetic condition that causes vision loss.
Methodology
The study used quantitative PCR, oligonucleotide microarray, and immunohistochemistry to analyze gene expression in mouse retinas.
Participant Demographics
C57/Bl6 wild-type and Rpe65−/− mice, aged 2 to 18 months.
Statistical Information
P-Value
p<0.01
Statistical Significance
p<0.01
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