Salmonella Typhimurium invasion of HEp-2 epithelial cells in vitro is increased by N-acylhomoserine lactone quorum sensing signals
2011
Salmonella Typhimurium and Quorum Sensing Signals
publication
Evidence: moderate
Author Information
Author(s): Live L Nesse, Kristin Berg, Lene K Vestby, Ingrid Olsaker, Berit Djønne
Primary Institution: Norwegian Veterinary Institute
Hypothesis
Do N-acylhomoserine lactone quorum sensing signals influence Salmonella Typhimurium's ability to invade epithelial cells?
Conclusion
Salmonella Typhimurium can increase its invasion of epithelial cells in response to certain quorum sensing signals.
Supporting Evidence
- The S. Typhimurium strain showed increased invasion with AHLs, while the sdiA mutant did not.
- Increased expression of rck and srgE genes was observed in the wild type strain with AHLs.
- The study indicates that AHLs may enhance Salmonella's invasiveness in the intestinal environment.
Takeaway
This study found that Salmonella can use signals from other bacteria to invade cells better, like getting a boost from friends.
Methodology
The study used a gentamycin invasion assay with HEp-2 cells and semi-quantitative PCR to measure gene expression.
Statistical Information
P-Value
p<0.05
Confidence Interval
95%
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
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