How Human Adenoviruses Use Heparan Sulfate Proteoglycans for Infection
Author Information
Author(s): Tuve Sebastian, Wang Hongjie, Jacobs Jeffrey D., Yumul Roma C., Smith David F., Lieber André
Primary Institution: University of Washington
Hypothesis
What are the cellular receptors used by human adenovirus serotypes 3 and 35?
Conclusion
The study found that human adenoviruses 3 and 35 utilize heparan sulfate proteoglycans as co-receptors for infection.
Supporting Evidence
- Ad3 and Ad35 interact with heparan sulfate proteoglycans (HSPGs) as co-receptors.
- HSPGs were not the main receptors but acted as low-affinity co-receptors for Ad3 and Ad35.
- The study identified a new CD46-independent infection pathway for Ad35 mediated by HSPGs.
Takeaway
This study shows that two types of viruses can use special molecules on our cells to help them get inside and cause infection.
Methodology
The researchers used recombinant fiber knobs and high-throughput receptor screening methods including mass spectrometry and glycan arrays.
Limitations
The study did not identify a high-affinity receptor for Ad3, which may limit understanding of its infection mechanism.
Digital Object Identifier (DOI)
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