Effects of Hormone Agonists on Sf9 Cells
Author Information
Author(s): Giraudo Maeva, Califano Jérôme, Hilliou Frédérique, Tran Trang, Taquet Nathalie, Feyereisen René, Le Goff Gaëlle
Primary Institution: Institut National de la Recherche Agronomique, UMR 1301 Interactions Biotiques et Santé Végétale, Centre National de la Recherche Scientifique, UMR 6243, Université de Nice Sophia Antipolis, Sophia-Antipolis, France
Hypothesis
How do methoxyfenozide and methoprene affect the proliferation and cell cycle of Sf9 cells?
Conclusion
Methoxyfenozide is more toxic than methoprene and induces different cell cycle arrests in Sf9 cells.
Supporting Evidence
- Methoxyfenozide was more toxic than methoprene in cell viability tests.
- Cell growth arrest occurred in the G2/M phase after methoprene treatment.
- Twenty-six genes were differentially expressed after methoxyfenozide treatment.
- Fifty-five genes were differentially expressed after methoprene treatment.
- Both insecticides inhibit cellular proliferation.
Takeaway
This study looked at how two insecticides that mimic hormones affect insect cells. One insecticide was found to be more harmful and caused cells to stop growing in different ways.
Methodology
The study used cell viability tests, flow cytometry, microarray experiments, and real-time quantitative PCR to analyze the effects of the insecticides.
Limitations
The study did not explore the long-term effects of the insecticides on cell viability and proliferation.
Statistical Information
P-Value
p<0.01
Statistical Significance
p<0.01
Digital Object Identifier (DOI)
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