Easy and Rapid Purification of Highly Active Nisin
Author Information
Author(s): André Abts, Mavaro Antonino, Stindt Jan, Bakkes Patrick J., Metzger Sabine, Driessen Arnold J. M., Smits Sander H. J., Schmitt Lutz
Primary Institution: Heinrich Heine University Düsseldorf
Hypothesis
An optimized purification protocol for nisin will yield a more active and less contaminated product.
Conclusion
The study presents a five-step purification method for nisin that significantly improves its bactericidal activity compared to traditional methods.
Supporting Evidence
- Nisin purified using the five-step method showed an IC50 of ~3 nM, a tenfold improvement over previous methods.
- The five-step NaCl elution effectively removed contaminants, resulting in a more active nisin preparation.
- Purified nisin exhibited high bactericidal activity against the nisin-sensitive L. lactis strain NZ9000.
Takeaway
The researchers found a better way to clean nisin, a natural antibiotic, so it works better against germs.
Methodology
Nisin was purified using a five-step NaCl elution process from cation exchange chromatography, followed by various assays to determine its activity.
Limitations
The presence of contaminants in nisin produced from L. lactis may affect its activity.
Digital Object Identifier (DOI)
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