Gene Discovery in Jatropha curcas for Biodiesel Production
Author Information
Author(s): Natarajan Purushothaman, Kanagasabapathy Deepa, Gunadayalan Gnanasekaran, Panchalingam Jasintha, Noopur Shree, Sugantham Priyanka Annabel, Singh Kavita Kumari, Madasamy Parani
Primary Institution: Genomics Laboratory, Department of Genetic Engineering, SRM University, Chennai, Tamil Nadu, India
Hypothesis
The study aims to isolate functional genes from Jatropha curcas to enhance its genetic improvement for biodiesel production.
Conclusion
A high-quality cDNA library was constructed, leading to the identification of 7009 unigenes that can be used for genetic improvement of Jatropha curcas.
Supporting Evidence
- The cDNA library contained about 1 × 10^6 clones with an average insert size of 2.1 kb.
- Contig analysis revealed 2258 contigs and 4751 singletons.
- Out of 7009 unigenes, 6233 were identified as potential full-length genes.
Takeaway
Scientists found many important genes in Jatropha curcas seeds that can help make better biodiesel and improve the plant's growth.
Methodology
A normalized and full-length enriched cDNA library was constructed from developing seeds, and 12,084 ESTs were sequenced to identify unigenes.
Digital Object Identifier (DOI)
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