Sensitivity of Human Dendritic Cells to Chemotherapy
Author Information
Author(s): Chao D, Bahl P, Houlbrook S, Hoy L, Harris A L, Austyn J M
Primary Institution: Nuffield Department of Surgery, John Radcliffe Hospital, Headington, Oxford OX3 9DU, UK
Hypothesis
Assessment of the chemosensitivity of dendritic cells (DC) may allow more rational development of combined chemotherapy and immunotherapy protocols.
Conclusion
Dendritic cells show varying sensitivity to different chemotherapy agents, suggesting that some agents may preserve their immunotherapeutic potential.
Supporting Evidence
- Dendritic cells were sensitive to doxorubicin with varying sensitivity between donors.
- The most extreme variation in sensitivity was seen for vinblastine.
- Etoposide and 5-fluorouracil showed relative resistance in dendritic cells.
Takeaway
This study looked at how well certain cancer drugs affect immune cells called dendritic cells, finding that some drugs are better for keeping these cells healthy.
Methodology
Human monocyte-derived dendritic cells were tested for survival after treatment with four different chemotherapy agents using the MTS assay.
Participant Demographics
Dendritic cells were prepared from three different donors.
Digital Object Identifier (DOI)
Want to read the original?
Access the complete publication on the publisher's website