Calpain Activator Dibucaine Induces Platelet Apoptosis
2011
Dibucaine Induces Platelet Apoptosis
publication
10 minutes
Evidence: moderate
Author Information
Author(s): Zhang Weilin, Liu Jun, Sun Ruichen, Zhao Lili, Du Juan, Ruan Changgeng, Dai Kesheng
Primary Institution: Beijing University of Aeronautics and Astronautics
Hypothesis
Does the calpain activator dibucaine induce platelet apoptosis?
Conclusion
Dibucaine induces platelet apoptosis and dysfunction without activating the platelets.
Supporting Evidence
- Dibucaine induced depolarization of mitochondrial inner transmembrane potential in platelets.
- Bax and Bak levels increased while Bcl-2 and Bcl-XL levels decreased in response to dibucaine.
- Caspase-3 was activated in platelets treated with dibucaine.
- Dibucaine did not induce platelet activation as shown by P-selectin expression.
- Platelet aggregation induced by ristocetin or α-thrombin was significantly inhibited by dibucaine.
Takeaway
Dibucaine is a substance that can make platelets die without making them work harder, which is important for understanding blood problems.
Methodology
The study involved incubating washed human platelets with dibucaine and analyzing various apoptotic markers and functions through flow cytometry and Western blotting.
Participant Demographics
Human platelets were used in the study.
Statistical Information
P-Value
p<0.05
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
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