Effects of Epstein-Barr Virus on Cell Damage
Author Information
Author(s): Gargouri Bochra, Nasr Rihab, Mseddi Malek, benmansour Riadh, Lassoued Saloua
Primary Institution: Unité de Biotechnologie et Pathologies, Institut Supérieur de Biotechnologie de Sfax, Tunisia
Hypothesis
Does the induction of the Epstein-Barr virus lytic cycle cause oxidative stress and damage to lipids, proteins, and DNA in lymphoblastoid B cell lines?
Conclusion
The study found that the induction of the EBV lytic cycle leads to significant oxidative modifications in lipids, proteins, and DNA in B95-8 and Raji cell lines.
Supporting Evidence
- Significant increases in conjugated dienes were observed in both B95-8 and Raji cell lines after EBV lytic cycle induction.
- Malondialdehyde adduct levels increased significantly in both cell lines post-induction.
- Protein carbonyl levels rose significantly in both cell lines after EBV lytic cycle induction.
- Protein thiol levels decreased significantly in both cell lines after induction.
- DNA fragmentation was detected in both B95-8 and Raji cell lines after EBV lytic cycle induction.
Takeaway
When the Epstein-Barr virus wakes up and starts to multiply, it can hurt the cells by damaging their important parts like fats, proteins, and DNA.
Methodology
The study measured levels of conjugated dienes, malondialdehyde adducts, protein carbonyls, protein thiols, and DNA fragmentation in two lymphoblastoid cell lines after inducing the EBV lytic cycle with TPA.
Participant Demographics
The study involved two lymphoblastoid B cell lines: B95-8 and Raji.
Statistical Information
P-Value
0.0001, 0.019, 0.008, 0.006, 0.003, 0.0039, 0.046, 0.002
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
Want to read the original?
Access the complete publication on the publisher's website