Effect of blood culture collection bundles on decreasing the contamination rate
2024

Reducing Blood Culture Contamination with a Collection Bundle

Sample size: 5034 publication 10 minutes Evidence: high

Author Information

Author(s): Doi Miki, Takesue Yoshio, Makino Miyuki, Kihara Yousuke, Tanikawa Akiko, Murakami Yasushi, Ogashiwa Hitoshi, Nakano Yukiko, Nakama Soichiro, Ueda Takashi, Nakajima Kazuhiko, Nozaki Yasuhiro

Primary Institution: Tokoname City Hospital, Tokoname, Aichi, Japan

Hypothesis

Can a bundled approach to blood culture collection decrease contamination rates?

Conclusion

The implementation of a bundled blood collection intervention significantly reduced the contamination rate from 2.0% to 1.0%.

Supporting Evidence

  • The contamination rate decreased significantly from 2.0% to 1.0% after the introduction of the bundle approach.
  • A significant decrease in contamination was observed for coagulase-negative Staphylococcus.
  • Blood collection through a line caused contamination in only one case during the study.
  • Chlorhexidine alcohol swabs were introduced for skin preparation, replacing povidone-iodine.
  • Use of a sterile blood transfer device was increased during the intervention period.
  • The study included a total of 5034 blood culture events over three years.

Takeaway

Using a special method to collect blood samples helped make sure fewer samples got contaminated, which is really important for getting the right test results.

Methodology

The study compared contamination rates before and after implementing a blood culture collection bundle that included specific skin disinfection and blood transfer techniques.

Potential Biases

The study was conducted at a single center, which may limit the generalizability of the results.

Limitations

The study did not collect data on patient comorbidities or antibiotic use, which could affect the findings.

Participant Demographics

Median age was 80 years, with a similar sex distribution (55% male) across both study periods.

Statistical Information

P-Value

p<0.001

Statistical Significance

p<0.001

Digital Object Identifier (DOI)

10.1371/journal.pone.0314649

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