Analyzing Binding Specificity of Epigenetic Reading Domains Using Peptide Arrays
Author Information
Author(s): Bock Ina, Kudithipudi Srikanth, Tamas Raluca, Kungulovski Goran, Dhayalan Arunkumar, Jeltsch Albert
Primary Institution: Jacobs University Bremen
Hypothesis
Celluspots peptide arrays can effectively screen the binding specificity of epigenetic reading domains to modified histone tails.
Conclusion
Celluspots peptide arrays are powerful screening tools for studying the specificity of reading domains binding to modified histone peptides.
Supporting Evidence
- Celluspots peptide arrays are an inexpensive and reliable method for screening protein interactions.
- The binding specificities of reading domains were consistent with existing literature.
- Secondary modifications can influence the binding affinity of reading domains to their target peptides.
- Control experiments confirmed the specificity of the binding results.
- Each reading domain was tested multiple times to ensure reliability of results.
Takeaway
Scientists used special arrays to see how proteins that read DNA modifications stick to different parts of DNA. They found that some changes nearby can help or stop this sticking.
Methodology
The study used Celluspots peptide arrays containing 384 histone peptides with 59 post-translational modifications to analyze the binding specificities of seven known epigenetic reading domains.
Limitations
The study primarily focused on initial screening and unexpected results need further validation through additional experiments.
Digital Object Identifier (DOI)
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