Rapid modification of the insect elicitor N-linolenoyl-glutamate on Nicotiana attenuata leaves
Author Information
Author(s): Arjen VanDoorn, Mario Kallenbach, Alejandro A. Borquez, Ian T. Baldwin, Gustavo Bonaventure
Primary Institution: Max Planck Institute for Chemical Ecology
Hypothesis
The metabolism of N-linolenoyl-glutamic acid (18:3-Glu) on Nicotiana attenuata leaves is mediated by a lipoxygenase-dependent mechanism.
Conclusion
The study found that the metabolism of 18:3-Glu results in the formation of oxidized forms that can elicit jasmonic acid biosynthesis and affect volatile emissions.
Supporting Evidence
- 70% of the initial 18:3-Glu was metabolized within 30 seconds of application.
- Plants silenced in NaLOX2 and NaLOX3 showed over 50% reduced rates of 18:3-Glu conversion.
- 13-oxo-13:2-Glu induced jasmonic acid accumulation similar to 18:3-Glu.
Takeaway
When a plant gets hurt, it quickly changes a special chemical from insects to help protect itself and call for help from other insects.
Methodology
The study analyzed the metabolism of 18:3-Glu on wounded leaf surfaces using LC-MS/MS and assessed the effects on jasmonic acid production and volatile emissions.
Limitations
The study primarily focused on a single elicitor and its derivatives, which may not represent all possible interactions in plant-insect dynamics.
Statistical Information
P-Value
p<0.05
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
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