A drop dispenser for simplifying on-farm detection of foodborne pathogens
Author Information
Author(s): Ranjbaran Mohsen, Kaur Simerdeep, Wang Jiangshan, Raut Bibek, Verma Mohit S.
Primary Institution: Purdue University, West Lafayette, Indiana, United States of America
Hypothesis
The drop dispenser can deliver precise sample volumes for nucleic acid testing in a user-friendly manner.
Conclusion
The drop dispenser developed in this study provides precise and reproducible drops for nucleic-acid testing, making it a promising tool for on-farm microbial risk assessments.
Supporting Evidence
- The drop dispenser was validated for nucleic acid testing using LAMP for detecting Escherichia coli O157.
- The device demonstrated similar performance to commercial pipettors in LAMP assays.
- The drop dispenser has a shelf life of approximately one month.
- The device can generate precise sample drops in volumes as small as a few micro-liters.
Takeaway
This study created a special drop dispenser that helps farmers easily test for germs in their food by giving them the right amount of sample every time.
Methodology
The drop dispenser was designed using 3D printing and tested for its ability to deliver precise sample volumes for LAMP assays.
Limitations
The device's performance is highly dependent on specific resin types and surface chemistry treatment, and it requires a rigorous washing protocol for optimal performance.
Statistical Information
P-Value
7.8×10^6 cells/mL
Statistical Significance
p<0.001
Digital Object Identifier (DOI)
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