Identification of a Bacillus thuringiensis Cry11Ba toxin-binding aminopeptidase from the mosquito, Anopheles quadrimaculatus
2006
Identifying a Mosquito Protein that Binds to a Bacillus thuringiensis Toxin
publication
Evidence: moderate
Author Information
Author(s): Abdullah Mohd Amir F, Valaitis Algimantas P, Dean Donald H
Primary Institution: The Ohio State University
Hypothesis
Do aminopeptidases from Anopheles quadrimaculatus bind to mosquitocidal Cry toxins?
Conclusion
An aminopeptidase from Anopheles quadrimaculatus mosquitoes is a specific binding protein for Bacillus thuringiensis Cry11Ba.
Supporting Evidence
- A 100-kDa protein with APN activity was isolated from the brush border membrane of Anopheles quadrimaculatus.
- Only Cry11Ba was able to bind the APNAnq 100 protein.
- The binding affinity of Cry11Ba to APNAnq 100 was determined to be tight with a KD of 0.56 nM.
Takeaway
Scientists found a protein in mosquitoes that sticks to a specific toxin from bacteria, which could help in understanding how to control mosquito populations.
Methodology
The study involved isolating brush border membrane vesicle proteins from Anopheles quadrimaculatus and testing their binding to various Cry toxins using surface plasmon resonance.
Digital Object Identifier (DOI)
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