Sterilization of Exopolysaccharides from Deep-Sea Bacteria
Author Information
Author(s): Emilie Rederstorff, Ahmed Fatimi, Corinne Sinquin, Jacqueline Ratiskol, Christophe Merceron, Claire Vinatier, Pierre Weiss, Sylvia Colliec-Jouault
Primary Institution: French Research Institute for Exploitation of the Sea (IFREMER)
Hypothesis
Which sterilization method is most appropriate for marine exopolysaccharides produced by deep-sea bacteria?
Conclusion
Ethylene oxide is the most suitable sterilization method for marine exopolysaccharides, causing the least damage to their properties.
Supporting Evidence
- Ethylene oxide sterilization showed minimal impact on the viscosity of HE800 EPS.
- Gamma and beta radiation caused significant decreases in the molecular weight of both EPSs.
- Cold plasma treatment resulted in a decrease in viscosity and molecular weight.
- All sterilization methods effectively eliminated bacteria from the EPSs.
Takeaway
Scientists tested different ways to sterilize substances made by deep-sea bacteria and found that one method, called ethylene oxide, is the best because it doesn't hurt the substances as much.
Methodology
The study compared the effects of different sterilization methods (ethylene oxide, gamma and beta radiation, cold plasma) on the physical and chemical properties of exopolysaccharides.
Limitations
The study does not address long-term effects of sterilization on biological activity.
Digital Object Identifier (DOI)
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