Deciphering protein–protein interactions. Part II. Computational methods to predict protein and domain interaction partners
2007

Predicting Protein Interactions

publication Evidence: moderate

Author Information

Author(s): Benjamin A. Shoemaker, Anna R. Panchenko

Primary Institution: Computational Biology Branch of the National Center for Biotechnology Information

Hypothesis

Can computational methods effectively predict protein and domain interaction partners?

Conclusion

Computational methods can complement experimental approaches to predict protein interactions, despite limitations in data completeness.

Supporting Evidence

  • High-throughput experimental methods produce large amounts of data that are often incomplete.
  • Computational methods can help validate experimental data and suggest potential protein interactions.
  • Different computational methods have varying degrees of success in predicting interactions.

Takeaway

Scientists can use computer programs to guess how proteins work together, even when they don't have all the information.

Methodology

The review discusses various computational methods for predicting protein interactions, including genomic inference, phylogenetic profiles, and classification methods.

Potential Biases

The reliance on existing experimental data may introduce biases based on the types of proteins studied.

Limitations

The methods rely on incomplete experimental data and assume independent interactions between domains.

Digital Object Identifier (DOI)

10.1371/journal.pcbi.0030043

Want to read the original?

Access the complete publication on the publisher's website

View Original Publication