Vascular endothelial growth factor expression is independent of hypoxia in human malignant glioma spheroids and tumours
2000
VEGF Expression in Gliomas and Hypoxia
publication
Author Information
Author(s): Parliament M B, Allalunis-Turner M J, Franko A J, Olive P L, Mandyam R, Santos C, Wolokoff B
Primary Institution: Cross Cancer Institute, Edmonton, Alberta, Canada
Hypothesis
Does oxygen regulate vascular endothelial growth factor (VEGF) expression in human glioma models?
Conclusion
VEGF expression is uniform in glioma regions regardless of hypoxia levels.
Supporting Evidence
- VEGF mRNA was found to be uniformly expressed across glioma spheroids.
- Regions of severe hypoxia did not correspond to areas of increased VEGF expression.
- VEGF expression levels varied among different glioma cell lines.
Takeaway
This study found that the amount of a protein called VEGF in brain tumors doesn't change much even when there's not enough oxygen around.
Methodology
In situ hybridization and immunohistochemistry were used to analyze VEGF expression in glioma models.
Digital Object Identifier (DOI)
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