Estrogen's Role in Breast Cancer Gene Regulation
Author Information
Author(s): Creighton Chad J, Cordero Kevin E, Larios Jose M, Miller Rebecca S, Johnson Michael D, Chinnaiyan Arul M, Lippman Marc E, Rae James M
Primary Institution: University of Michigan Medical Center
Hypothesis
Do genes regulated by estrogen in vitro also show similar regulation in vivo in breast tumors?
Conclusion
The study validates that genes regulated by estrogen in vitro are similarly regulated in vivo in breast tumors.
Supporting Evidence
- Over 40% of genes regulated by estrogen in vitro were also regulated in vivo.
- E2-regulated genes were co-expressed with ERĪ± in human tumors.
- The study found significant enrichment for transcriptional targets of the myc oncogene.
Takeaway
This study shows that the way estrogen affects genes in lab-grown breast cancer cells is similar to how it affects genes in actual tumors.
Methodology
DNA microarray-based gene expression profiles were generated from breast cancer cell lines and xenografts.
Potential Biases
Potential biases in gene selection and interpretation of microarray data.
Limitations
The study may not account for all variables affecting gene expression in human tumors.
Participant Demographics
The study involved athymic nude mice and human breast tumor samples.
Statistical Information
P-Value
p<0.05
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
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