Fur Activates hilD Expression in Salmonella enterica
Author Information
Author(s): Teixidó Laura, Carrasco Begoña, Alonso Juan C., Barbé Jordi, Campoy Susana
Primary Institution: Departament de Genètica i de Microbiologia, Facultat de Biociències. Universitat Autònoma de Barcelona, Bellaterra, Spain
Hypothesis
Fur modulates the expression of the S. enterica hilD gene through direct interaction with its promoter.
Conclusion
Fur, in its metal-bound form, directly stimulates the expression of hilD in Salmonella enterica.
Supporting Evidence
- Fur binds specifically to an AT-rich region upstream of the hilD promoter.
- In vivo experiments showed that Fur activates hilD expression.
- In vitro experiments confirmed that Fur·Mn2+ is sufficient to increase hilD transcription.
- Mutations in the Fur binding site resulted in impaired hilD expression.
Takeaway
Fur is a protein that helps Salmonella bacteria know when to turn on a gene that helps them invade our intestines, especially when there's enough iron around.
Methodology
The study used in vivo and in vitro experiments to analyze the binding of Fur to the hilD promoter and its effect on gene expression.
Digital Object Identifier (DOI)
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