Impact of Perfusion Parameters on Organ Weight Gain in Ex Vivo Perfusion
Author Information
Author(s): Marlar Riley, Abbas Fuad, Obeid Rommy, Frisbie Sean, Ghazoul Adam, Rezaee Ava, Sims Jack, Rampazzo Antonio, Bahar Bassiri Gharb
Primary Institution: Cleveland Clinic
Hypothesis
There is a significant relationship between specific parameters during ex vivo machine perfusion and perfusion outcomes.
Conclusion
Oxygen carriers, low flow rates, and normothermic perfusate temperature appear to improve outcomes in ex vivo machine perfusion.
Supporting Evidence
- Red blood cell‐based perfusates resulted in significantly lower weight gain compared to acellular perfusates without oxygen carriers.
- Hemoglobin‐based oxygen carriers resulted in significantly lower weight gain compared to acellular perfusates.
- Normothermic perfusion led to the least weight gain compared to hypothermic and subnormothermic conditions.
- There was a positive correlation between flow rate and weight gain.
Takeaway
This study found that using certain fluids and temperatures during organ preservation can help keep organs healthier for transplant.
Methodology
A meta-analysis of literature was conducted, analyzing 44 articles on ex vivo machine perfusion.
Potential Biases
Potential bias due to variability in perfusion protocols across studies.
Limitations
The study's findings depend on the quality of the included studies and their reported outcomes.
Participant Demographics
Studies included various species: pig, rat, dog, human, and rabbit.
Statistical Information
P-Value
p<0.001
Statistical Significance
p<0.001
Digital Object Identifier (DOI)
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