Study of Mouse Cells Transformed by HPV16
Author Information
Author(s): Šmahel M, Sobotková E, Bubeník J, Šímová J, Žák R, Ludvíková V, Hájková R, Kovařík J, Jelínek F, Povýšil C, Marinov J, Vonka V
Primary Institution: Department of Experimental Virology, Institute of Hematology and Blood Transfusion
Hypothesis
Can mouse cells transformed with HPV16 and H-ras oncogenes serve as a model for studying gene therapy of cancers associated with human papillomaviruses?
Conclusion
The study found that the transformed mouse cells could be used as a model for studying immune reactions against HPV16-associated tumors.
Supporting Evidence
- The transformed cells did not express MHC class I and II molecules.
- Spontaneous metastases were observed in lymphatic nodes and lungs.
- Lethally irradiated MK16/1/IIIABC cells provided some protection against homologous cell challenges.
Takeaway
Researchers made special mouse cells using a virus to help understand how to treat certain cancers. These cells can help scientists learn how the body fights these cancers.
Methodology
Mouse kidney cells were transformed using plasmids carrying HPV16 E6/E7 oncogenes and activated H-ras oncogenes.
Statistical Information
P-Value
p<0.03
Statistical Significance
p<0.03
Digital Object Identifier (DOI)
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