Phosphoglycerate mutase, 2,3-bisphosphoglycerate phosphatase, creatine kinase and enolase activity and isoenzymes in breast carcinoma
2000
Enzyme Activity in Breast Cancer
Sample size: 12
publication
Author Information
Author(s): Durany N, Joseph J, Jimenez O M, Climent F, Fernández P L, Rivera F, Carreras J
Primary Institution: Unit of Biochemistry, IDIBAPS, Faculty of Medicine, University of Barcelona
Hypothesis
The study investigates the differences in enzyme activity and isoenzyme distribution between normal breast tissue and breast carcinoma.
Conclusion
Breast carcinoma tissue shows higher levels of certain enzyme activities compared to normal breast tissue.
Supporting Evidence
- Tumour tissue had higher phosphoglycerate mutase and enolase activity than normal tissue.
- Creatine kinase activity was higher in seven out of 12 tumours.
- 2,3-bisphosphoglycerate phosphatase activity was lower in tumours.
- Greater changes in enzyme activity were observed in oestrogen receptor-negative/progesterone receptor-negative breast carcinomas.
Takeaway
This study looked at how some enzymes behave differently in breast cancer compared to normal breast tissue.
Methodology
The study compared enzyme activities and isoenzyme distributions in normal and cancerous breast tissues using electrophoresis.
Digital Object Identifier (DOI)
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