THE EXCHANGE RATE OF THE THIOL LIGAND OF iPr3PAuSATg WITH THE CYS-34 RESIDUE OF BOVINE SERUM ALBUMIN
Thiol Ligand Exchange in a Drug and Serum Albumin
publication
Author Information
Author(s): J. Xiao, C. Frank Shaw III
Primary Institution: University of Wisconsin-Milwaukee
Hypothesis
The study investigates the rate of thiol ligand exchange between a drug and the Cys-34 residue of serum albumin.
Conclusion
The forward and reverse rates of thiol ligand exchange are similar, but the forward reaction is faster due to the higher affinity of Cys-34 for gold(I).
Supporting Evidence
- The forward rate of thiol ligand exchange is 0.12X10^-3 M^-1s^-1.
- The reverse rate of thiol ligand exchange is 0.39X10^-4 M^-1s^-1.
- The equilibrium constant for the reaction is calculated to be 3.1.
- The equilibrium constant measured by liquid chromatography is 3.0.
Takeaway
This study looks at how a drug interacts with a protein in the body, showing that the drug can swap parts with the protein, which happens faster in one direction.
Methodology
A 31P NMR saturation transfer study was performed to investigate the thiol ligand exchange.
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