Isolation and functional characterization of a cDNA coding a hydroxycinnamoyltransferase involved in phenylpropanoid biosynthesis in Cynara cardunculus L.
2007
Study of a Gene Involved in Chlorogenic Acid Production in Globe Artichoke
publication
Evidence: moderate
Author Information
Author(s): Cinzia Comino, Sergio Lanteri, Ezio Portis, Alberto Acquadro, Annalisa Romani, Alain Hehn, Romain Larbat, Frédéric Bourgaud
Primary Institution: University of Torino
Hypothesis
The study aims to understand the synthesis of chlorogenic acid (CGA) in Cynara cardunculus L.
Conclusion
The study confirms that the hydroxycinnamoyltransferase (HCT) gene is involved in the biosynthesis of CGA in globe artichoke.
Supporting Evidence
- The gene sequence isolated shares 84% amino acid identity with a tobacco gene involved in CGA biosynthesis.
- Heterologous expression in E. coli confirmed the enzyme's ability to synthesize chlorogenic acid.
- Variable levels of HCT expression were correlated with CGA content in different C. cardunculus subspecies.
Takeaway
Researchers found a gene in artichokes that helps make a healthy compound called chlorogenic acid, which is good for our bodies.
Methodology
The study involved isolating a gene sequence using PCR and heterologous expression in E. coli to assess enzyme activity.
Digital Object Identifier (DOI)
Want to read the original?
Access the complete publication on the publisher's website