Measuring Bactericidal/Permeability-Increasing Protein in Human Serum
Author Information
Author(s): J.-O. Hiiggblom, A. B. Jokilammi-Siltanen, H. Peuravuori, T. J. Nevalainen
Primary Institution: University of Turku
Hypothesis
Can a time-resolved fluoroimmunoassay reliably measure the concentration of BPI in human serum?
Conclusion
The study demonstrates that BPI can be reliably quantified in human serum using a time-resolved fluoroimmunoassay.
Supporting Evidence
- The mean concentration of BPI in serum samples was 28.3 µg/l.
- The mean concentration of BPI in plasma samples was 52.3 µg/l.
- There was a positive correlation between BPI concentration and white blood cell count.
- There was a positive correlation between BPI concentration and C-reactive protein levels.
Takeaway
This study found a way to measure a protein that helps fight infections in the blood, which can help doctors understand how sick someone is.
Methodology
A time-resolved fluoroimmunoassay was developed to measure BPI concentrations in serum and plasma samples from hospitalized patients.
Limitations
No correlation was found between BPI values and white blood cell counts in plasma samples.
Participant Demographics
42 women and 48 men, average age 61 years (range 14-93 years).
Statistical Information
P-Value
p < 0.0001
Statistical Significance
p < 0.0001
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