Microsatellites in Phytophthora Species
Author Information
Author(s): Diana P. Garnica, Andrés M. Pinzón, Lina M. Quesada-Ocampo, Adriana J. Bernal, Emiliano Barreto, Niklaus J. Grünwald, Silvia Restrepo
Primary Institution: Universidad de Los Andes
Hypothesis
How do microsatellites vary in frequency and distribution among different Phytophthora species?
Conclusion
The study found that trinucleotide repeats are the most common microsatellites in Phytophthora species, with a small proportion of primers transferable between species.
Supporting Evidence
- The most common SSRs were trinucleotide repeat units with low repeat numbers.
- A proportion of 7.5% of primers could be transferred with 90% similarity between at least two species of Phytophthora.
- The SSR survey results were deposited in a public database.
Takeaway
Scientists looked at tiny repeating DNA sequences in some plant diseases and found that most of them are made up of three letters repeated over and over again.
Methodology
An in silico approach was used to analyze the frequency and distribution of microsatellites in transcript sequences from Phytophthora infestans, P. sojae, and P. ramorum.
Limitations
The study primarily focused on transcript sequences and may not represent the full genomic context of microsatellites.
Statistical Information
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
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