c-Myc activates BRCA1 gene expression through distal promoter elements in breast cancer cells
2011
c-Myc Activates BRCA1 Gene Expression in Breast Cancer Cells
publication
Evidence: moderate
Author Information
Author(s): Chen Yinghua, Xu Jinhua, Borowicz Stanley, Collins Cindy, Huo Dezheng, Olopade Olufunmilayo I
Primary Institution: University of Chicago
Hypothesis
Does c-Myc regulate BRCA1 expression through distal promoter elements in breast cancer cells?
Conclusion
c-Myc activates BRCA1 expression through specific E boxes in the distal BRCA1 promoter region.
Supporting Evidence
- Depletion of c-Myc reduced BRCA1 mRNA and protein levels.
- Ectopic expression of c-Myc increased BRCA1 promoter activity.
- c-Myc bound to E boxes in the BRCA1 promoter in vivo.
- Nucleotide substitutions in E boxes abrogated c-Myc-dependent activation.
- Reduced BRCA1 expression due to c-Myc depletion impaired DNA repair activity.
Takeaway
c-Myc helps a gene called BRCA1 work better, which is important for fixing DNA in breast cancer cells.
Methodology
The study used cultured breast cancer cells, siRNA to deplete c-Myc, and various assays to measure BRCA1 expression and activity.
Statistical Information
P-Value
p<0.01
Statistical Significance
p<0.01
Digital Object Identifier (DOI)
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